stimulator syntech stimulus controller cs55 v2 (Syntech GmbH)
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Stimulator Syntech Stimulus Controller Cs55 V2, supplied by Syntech GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stimulator+syntech+stimulus+controller+cs55+v2/stimulus+controller+cs+55/pmc09492728-290-1-2
Average 90 stars, based on 1 article reviews
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1) Product Images from "Internal state affects local neuron function in an early sensory processing center to shape olfactory behavior in Drosophila larvae"
Article Title: Internal state affects local neuron function in an early sensory processing center to shape olfactory behavior in Drosophila larvae
Journal: Scientific Reports
doi: 10.1038/s41598-022-20147-1
Figure Legend Snippet: Odor responses of Keystone-LN in fed and starved larvae. ( A ). Schematic of in-vivo calcium imaging set-up. The odor stimulator consists of 3 air outlets, one for the carrier gas flow and two for the pulse flow. Under no stimulus conditions, airflow from outlets 1 and 3 are constant. When a stimulus is triggered, the airflow from outlet 1 is switched to outlet 2, which puffs air through an odor-containing syringe for 1 s, while carrier flow remains constant. Odor responses are recorded from the neurites of Keystones in the AL (Keystone-gal4; UAS-GCaMP6m) using a spinning disk confocal microscope. ( B ) Representative odor-evoked calcium responses in the neurites of Keystone-LNs to the indicated odors diluted in paraffin oil. ( C ) The top panels show maximum projection images of baseline GCaMP fluorescence in Keystone-LN neurites in the antennal lobe. The bottom heatmaps represent the responses of Keystone-LN neurites to the indicated odors under fed and starved conditions. Each row corresponds to responses from an individual larva. The black bar indicates when the 1-s odor stimulus was applied. ( D ) Box plots showing solvent corrected response amplitudes with min–max normalization (see “ ”) comparing responses to different odors in fed and starved states. Mann–Whitney U -test was used to test differences between fed and starved conditions. No statistically significant differences were found for any of the odors tested.
Techniques Used: In Vivo, Imaging, Microscopy, Fluorescence, Solvent, MANN-WHITNEY